Emergency services were called to the fire at Fife Zoo in Cupar this afternoon shortly after 12pm. Crews responded as pictures on social media showed smoke filling the skyline.
The fire reportedly started in a soft play building with four fire crews in currently in attendance.
All visitors have been evacuated from the zoo.
Meanwhile, zookeepers safely evacuated marmosets, who were in an enclosure closest to the fire.
A spokesperson for Scottish Fire and Rescue Service, said: “We received the call around 12.11pm and are in attendance.”
One eyewitness told Fife Free Press: “There is smoke and flames pouring out of the soft play building.
“Luckily the keepers were able to evacuate the marmosets safely. Their enclosure was closest to the site of the fire.
“It looked at one point like the firefighters had got the blaze under control, but the flames have now taken hold of the building.
One wrote on social media: “Just passed from a distance and saw the smoke. It is so big!”
Another said: “Hope animals and staff are ok. What a shame they just managed to get it reopened. Thinking of everyone involved.”
A final person added: “This is such a shame after all the hard work they have put in to get it opening.. hope the animals are all ok and the owners and staff are safe.
A statement from Fife Zoo, said: “All humans and animals are safe. All emergency teams are on site to contain the fire. Thank you for the concern”